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er gfp addgene plasmid  (Addgene inc)


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    Addgene inc er gfp addgene plasmid
    Er Gfp Addgene Plasmid, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 51 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/er+gfp+addgene+plasmid/LAMP1-GFP+(Plasmid+%2316290)/pm41838720-407-28-29
    Average 93 stars, based on 51 article reviews
    er gfp addgene plasmid - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Virus:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Plasmid Preparation:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Recombinant:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Transfection:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Modification:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Saline:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Negative Control:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    esiRNA:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..

    Software:

    Article Title: Mitochondria acidify lysosomes through membrane contacts.
    Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-VPS39 Santa Cruz Biotechnology Cat# sc-514762; RRID:AB_2687985 Anti-GAPDH Cell Signaling Technology Cat# 14C10 Bacterial and virus strains LAMP1-GFP Addgene plasmid Cat# 16290 ER-GFP Addgene plasmid Cat# 56482 ER-mCherry Addgene plasmid Cat# 55041 LAMP–mCherry–CRY2 Addgene plasmid Cat# 102249 TOM20–CIB–GFP Addgene plasmid Cat# 117242 sfGFP-LAMP1-mCherry Addgene plasmid Cat# 164477 Chemicals, peptides, and recombinant proteins MitoTracker Deep Red FM Thermo Fisher Scientific Cat# M22426 LysoTracker Green DND-26 Thermo Fisher Scientific Cat# L7526 LysoTracker red DND–99 Thermo Fisher Scientific Cat# L7528 LysoTracker Deep Red Thermo Fisher Scientific Cat# L12492 pHrodo Deep Red Thermo Fisher Scientific Cat# P35360 Sucrose Thermo Fisher Scientific Cat# A15583-36 pHLys Red Dojindo Molecular Technologies N/A Hydrazine hydrate Aladdin N/A Tetrahydrofuran Energy Chemical N/A 2-Hydroxy-1-naphthaldehyde Energy Chemical N/A 4-(4-Methylpiperazin-1-yl) benzaldehyde Energy Chemical N/A Methanol Energy Chemical N/A 2,4-Dibromoquinoline AmBeed N/A 4-Ethynyl-N,N-dimethylaniline Sigma N/A Methyl trifluoromethanesulfonate Sigma N/A Critical commercial assays Turbofect transfection solution Thermo Fisher Scientific Cat# 0531 Lipofectamine 3000 transfection reagent Thermo Fisher Scientific Cat# L3000015 Dulbecco’s modified Eagle’s medium Thermo Fisher Scientific Cat# 11965092 Fetal bovine serum Thermo Fisher Scientific Cat# 26140079 Penicillin– streptomycin Thermo Fisher Scientific Cat# 15140163 Phosphate-buffered saline GE Healthcare Life Sciences Cat# SH30256.01 Experimental models: Cell lines HeLa Chen et al.7 N/A PC12 Pei et al.31 N/A N418 Manupati et al.32 N/A MCF-7 Melkoumian et al.33 N/A Oligonucleotides Universal Negative Control siRNA Sigma-Aldrich Cat# SIC001 VPS39 esiRNA Sigma-Aldrich Cat# EHU066081 VPS39 siRNA Thermo Fisher Scientific ID# s23601 Software and algorithms Prism GraphPad v.8 (Continued on next page) 12 Cell Reports 45, 117112, March 24, 2026 ..



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    KEY RESOURCES TABLE

    Journal: Cancer cell

    Article Title: Widespread selection for oncogenic mutant allele imbalance in cancer

    doi: 10.1016/j.ccell.2018.10.003

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rabbit polyclonal, Phospho-MEK (Ser217/221) Cell Signal Cat#9121; RRID: AB_331649 Rabbit polyclonal, Anti-MEK Cell Signal Cat#9122; RRID: AB_823567 Rabbit polyclonal, Phospho ERK (Thr202/Tyr204) Cell Signal Cat#9109; RRID: AB_331646 Rabbit polyclonal, Anti-ERK Cell Signal Cat#9102; RRID: AB_330744 Rabbit monoclonal, Anti-GFP Cell Signal Cat#2956; RRID: AB_1196615 Rabbit monoclonal, Anti-GAPDH Cell Signal Cat#2118; RRID: AB_561053 Biological Samples MSK-IMPACT Zehir et al. 2017 N/A Chemicals, Peptides, and Recombinant Proteins pEGPF-N1-MEK1 Addgene Cat#14746 3X-ERE-TATA-luciferase reporter Addgene Cat#11354 pRL-TK Renilla luciferase plasmid Promega Cat#E2231 HA-tagged WT ERα This study N/A pcDNA3.1 (EV) Thermo Fisher Cat#V79020 HA-tagged ERα Y537S This study N/A HA-tagged ERα D538G This study N/A Critical Commercial Assays QuikChange II XL Site-Directed Mutagenesis Kit Stratagene Cat#200521 SuperSignal West Pico Plus Chemiluminescent Thermo Fisher Cat#34577 Lipofectamine 2000 Transfection Reagent Invitrogen Cat#11668027 Dual-Luciferase Reporter Assay System Promega Cat#E1910 Deposited Data Prospective DNA sequencing This study Accession number for the prospective sequencing data reported in this study is EVA: PRJEB28874, https://www.ebi.ac.uk/eva/?eva-study=PRJEB28874 Experimental Models: Cell Lines SKBr3 ATCC HTB30 HEK-293H ATCC CRL-1573 Software and Algorithms R for Statistical Computing R Foundation http://www.r-project.org FACETS Shen, et al. 2016 http://github.com/mskcc/facets FACETS-suite This study http://github.com/mskcc/facets-suite Other BAM files for TCGA exomes TCGA https://cancergenome.nih.gov Open in a separate window KEY RESOURCES TABLE Mutant allele imbalance affects ~half of all oncogenic driver mutations in cancer Positive and negative selection shapes the allelic configuration of mutant oncogenes Mutant dosage gains and stoichiometric changes drive fitness and growth advantages Mutant oncogene zygosity dictates prognosis and enhanced therapeutic sensitivity Bielski et al. describe widespread allele imbalance in cancer, which is selected for via modest dosage increases of gain-of-fitness mutations and can be driven by antecedent oncogenic mutations.

    Techniques: Recombinant, Luciferase, Plasmid Preparation, Mutagenesis, Transfection, Reporter Assay, DNA Sequencing, Sequencing, Software